Part:BBa_K209497:Design

pcDNA3.1(+)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 991
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2313
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal NheI site found at 895
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2313
  Illegal NotI site found at 978
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal BglII site found at 12
  Illegal BamHI site found at 929
  Illegal XhoI site found at 985
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 991
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2313
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 952
  Illegal XbaI site found at 991
  Illegal SpeI site found at 249
  Illegal SpeI site found at 935
  Illegal PstI site found at 957
  Illegal PstI site found at 2313
  Illegal NgoMIV site found at 1423
  Illegal NgoMIV site found at 2764
  Illegal NgoMIV site found at 3047
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 882
  Illegal BsaI.rc site found at 4572
  Illegal SapI site found at 3492
  Illegal SapI.rc site found at 2613
  Illegal SapI.rc site found at 2823
  

Design Notes

Not a designed plasmid.

Source

Invitrogen